E-IR-R109-10mL | Proteinase K Reagent(10 mg/mL) Clinisciences

Product Introduction	Proteinase K is a kind of high activity Proteinase of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on. Enzyme activity, > 30 U/mg. At 37°C, the amount of Proteinase K that can produce amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive in one minute with hemoglobin as the substrate is defined as a unit of Proteinase K activity. The effective pH range of Proteinase K is pH4.0~12.5, and the optimal pH range is pH7.5~8.0. The optimum reaction temperature of Proteinase K is 65°C, but at 65°C or higher, Proteinase K can also degrade rapidly. It is suggested that the optimum reaction temperature is 50~55°C.
Applications	IF, FCM
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
Cautions	1. This product couldn’t be used for clinical diagnosis or treatment, food or medicine, and can’t be stored in residence. 2. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

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Proteinase K Reagent(10 mg/mL)