RAB5A polyclonal antibody (ATTO 488)

Product Description

Rabbit polyclonal antibody raised against synthetic peptide of RAB5A.

Immunogen

A synthetic peptide conjugated with KLH corresponding to human RAB5A.

Host

Rabbit

Reactivity

Bovine, Human, Monkey, Mouse, Rat

Specificity

The antibody recognizes 26 kDa Rab5 of the above species origins.

Form

Liquid

Conjugation

ATTO 488

Purification

Protein A purification

Recommend Usage

Immunocytochemistry/Immunofluorescence (1:80)
Immunohistochemistry (1:100)
Western blot (1:1000)
The optimal working dilution should be determined by the end user.

Storage Buffer

In PBS (50% glycerol, 0.09% sodium azide).

Storage Instruction

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Application Data with Unconjugated Antibody

Western Blot (Cell lysate)

Western blot analysis of human cell line mix with RAB5A polyclonal antibody (PAB29000) at 1:1000 dilution.

Immunofluorescence

Immunofluorescent staining of Bouin's fixed paraffin-embedded backskin sections of transgenic mice with RAB5A polyclonal antibody (PAB29000).