Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Sirtinol [410536-97-9]

Sirtinol [410536-97-9]

Imprimer Imprimer
Identifiez-vous

Référence HY-13515-50mg

Conditionnement : 50mg

Marque : MedChemExpress

Demander plus d'informations

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

Sirtinol is a sirtuin (SIRT) inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively.

Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.

Sirtinol Chemical Structure

Sirtinol Chemical Structure

CAS No. : 410536-97-9

Size Prix Stock Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
5 mg En stock
10 mg En stock
50 mg En stock
100 mg En stock
200 mg   Obtenir un devis  
500 mg   Obtenir un devis  
or Bulk Inquiry

* Veuillez sélectionner la quantité avant d'ajouter des articles.

This product is a controlled substance and not for sale in your territory.

Based on 15 publication(s) in Google Scholar

    Sirtinol purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 May 1;9(5):559.  [Abstract]

    The inhibitory effect of CHES1 on transcription activity of ERα Is diminished in MCF7 cells when treated with Sirtinol (the SIRT1 inhibitor).

    Sirtinol purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2018 Feb;439(1-2):213-223.  [Abstract]

    Western blot analysis in cells treated with 10 μM Sirtinol prior to incubation with 200 μM Resveratrol (RES) calculated from density values of specific protein bands/GAPDH density values and expressed as a percentage of the control.
    Description

    Sirtinol is a sirtuin (SIRT) inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively[1][2][3][4].

    IC50 & Target[4]

    ySir2

    48 μM (IC50)

    hSIRT2

    57.7 μM (IC50)

    hSIRT1

    131 μM (IC50)

    Cellular Effect
    Cell Line Type Value Description References
    Hs 683 IC50
    33.9 μM
    Compound: 2
    Antiproliferative activity against human Hs683 cells after 72 hrs by MTT assay
    Antiproliferative activity against human Hs683 cells after 72 hrs by MTT assay
    		[PMID: 28475330]
    U-373MG ATCC IC50
    39.3 μM
    Compound: 2
    Antiproliferative activity against human U373 cells after 72 hrs by MTT assay
    Antiproliferative activity against human U373 cells after 72 hrs by MTT assay
    		[PMID: 28475330]
    In Vitro

    Sirtinol reduces the growth of MCF-7 cells in a concentration- and time-dependent manner. The IC50 values of sirtinol are 48.6 μM and 43.5 μM after 24 and 48 h of treatment, respectively. Sirtinol significantly decreases SIRT1 expression and increases the acetylated p53 level[1]. Sirtinol attenuates the proliferation and induces apoptosis of nonsmall cell lung cancer (NSCLC) H1299 cells and causes the significantly increased level of FoxO3a, a proapoptotic transcription factor targeted by Sirt1[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sirtinol Related Antibodies
    In Vivo

    Sirtinol has anti-inflammatory effects through direct inhibition of HNE activity and attenuates HNE-induced and LPS-mediated tissue or organ injury[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Masse moléculaire

    394.47

    Formule

    C26H22N2O2
    CAS No.

    410536-97-9

    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(NC(C1=CC=CC=C1)C)C2=CC=CC=C2/N=C/C3=C4C=CC=CC4=CC=C3O
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvant et solubilité
    In Vitro: 

    DMSO : 10 mg/mL (25.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5350 mL 12.6752 mL 25.3505 mL
    5 mM 0.5070 mL 2.5350 mL 5.0701 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1 mg/mL (2.54 mM); Clear solution

      This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureté et documentation

    Purity: ≥98.0%

    SDS (392 KB)

    COA (251 KB) HNMR (261 KB)

    Instruction de manipulation (2659 KB)
    Références

    • [1]. Wang J, et al. Sirtinol, a class III HDAC inhibitor, induces apoptotic and autophagic cell death in MCF-7 human breast cancer cells. Int J Oncol. 2012 Sep;41(3):1101-1109.  [Content Brief]

      [2]. Fong Y, et al. The antiproliferative and apoptotic effects of sirtinol, a sirtuin inhibitor on human lung cancer cells by modulating Akt/β-catenin-Foxo3a axis. ScientificWorldJournal. 2014;2014:937051.  [Content Brief]

      [3]. Mai A, et al. Design, synthesis, and biological evaluation of sirtinol analogues as class III histone/protein deacetylase (Sirtuin) inhibitors. J Med Chem. 2005 Dec 1;48(24):7789-95.  [Content Brief]

      [4]. Tsai YF, et al. Sirtinol inhibits neutrophil elastase activity and attenuates lipopolysaccharide-mediated acute lung injury in mice. Sci Rep. 2015 Feb 10;5:8347.  [Content Brief]

    Test cellulaire
    [2]

    Sirtinol is dissolved in 100% DMSO at concentration of 10 mM and stored at −20°C until use. The cell proliferation of H1299 cells is determined by trypan blue dye exclusion assay. Human nonsmall cell lung cancer (NSCLC) cells are seeded in 12-well plates and treated with indicated concentrations of sirtinol (0, 10, 20, and 50 μM) for 24 h and 48 h, respectively. After incubation, the cells are stained by 0.2% trypan blue and counted by Countess[2].

    MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
    Administration animale
    [3]

    Mice: 30 male mice (20–25 g, 7–8 weeks old) are used in this model. Briefly, mice are randomly divided into five groups; then mice are intraperitoneally injected with 50 μL DMSO (vehicle group) or sirtinol (2.5 or 5.0 mg/kg body weight). After 1 h, paw inflammation is induced . The thickness of the paw is measured before and after HNE or saline injection[3].

    MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
    Références

    • [1]. Wang J, et al. Sirtinol, a class III HDAC inhibitor, induces apoptotic and autophagic cell death in MCF-7 human breast cancer cells. Int J Oncol. 2012 Sep;41(3):1101-1109.  [Content Brief]

      [2]. Fong Y, et al. The antiproliferative and apoptotic effects of sirtinol, a sirtuin inhibitor on human lung cancer cells by modulating Akt/β-catenin-Foxo3a axis. ScientificWorldJournal. 2014;2014:937051.  [Content Brief]

      [3]. Mai A, et al. Design, synthesis, and biological evaluation of sirtinol analogues as class III histone/protein deacetylase (Sirtuin) inhibitors. J Med Chem. 2005 Dec 1;48(24):7789-95.  [Content Brief]

      [4]. Tsai YF, et al. Sirtinol inhibits neutrophil elastase activity and attenuates lipopolysaccharide-mediated acute lung injury in mice. Sci Rep. 2015 Feb 10;5:8347.  [Content Brief]

    • [1]. Wang J, et al. Sirtinol, a class III HDAC inhibitor, induces apoptotic and autophagic cell death in MCF-7 human breast cancer cells. Int J Oncol. 2012 Sep;41(3):1101-1109.

      [2]. Fong Y, et al. The antiproliferative and apoptotic effects of sirtinol, a sirtuin inhibitor on human lung cancer cells by modulating Akt/β-catenin-Foxo3a axis. ScientificWorldJournal. 2014;2014:937051.

      [3]. Mai A, et al. Design, synthesis, and biological evaluation of sirtinol analogues as class III histone/protein deacetylase (Sirtuin) inhibitors. J Med Chem. 2005 Dec 1;48(24):7789-95.

      [4]. Tsai YF, et al. Sirtinol inhibits neutrophil elastase activity and attenuates lipopolysaccharide-mediated acute lung injury in mice. Sci Rep. 2015 Feb 10;5:8347.

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5350 mL 12.6752 mL 25.3505 mL 63.3762 mL
    5 mM 0.5070 mL 2.5350 mL 5.0701 mL 12.6752 mL
    10 mM 0.2535 mL 1.2675 mL 2.5350 mL 6.3376 mL
    15 mM 0.1690 mL 0.8450 mL 1.6900 mL 4.2251 mL
    20 mM 0.1268 mL 0.6338 mL 1.2675 mL 3.1688 mL
    25 mM 0.1014 mL 0.5070 mL 1.0140 mL 2.5350 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Sirtinol Related Classifications

    • Cell Cycle/DNA Damage Epigenetics Autophagy Apoptosis
    • Sirtuin Autophagy Apoptosis
    Help & FAQs

    • Keywords:

      Sirtinol410536-97-9SirtuinAutophagyApoptosisInhibitorinhibitorinhibit

    Vous serez peut-être également intéressé par les produits suivants :



    Référence
    Description
    Cond.
    Prix HT
    HY-101103-10g
    HP-β-CD [128446-35-5]
     10g