SK-MEL-1
Référence 300424
Conditionnement : 1cryovial
Marque : CLS Cell Lines Service
SK-MEL-1 Cells
General information
Description | This cell line was established in 1966 by F. Oettgen and associates using cells from the thoracic duct of a patient. Pigment granules relating both to synthesis and to phagocytosis are present. According to our sequencing, WB and PCR results this cell line carries a BRAF V600E mutation. Cells are N-Ras wildtype. |
---|---|
Organism | Human |
Tissue | Skin |
Disease | Melanoma |
Metastatic site | Thoracic lymph duct |
Synonyms | SK-Mel-1, SK Mel 1, SK-Mel 1, SK-Mel1, SKMEL-1, SkMEL-1, SKMEL1, SK 1 |
Characteristics
Age | 29 years |
---|---|
Gender | Male |
Ethnicity | Caucasian |
Morphology | Spherical |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | SK-MEL-1 (Cytion catalog number 300424) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Antigen expression | Blood Type A, Rh+. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. |
---|---|
Isoenzymes | PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, |
Tumorigenic | Yes, in nude mice. Forms pigmented malignant melanomas. Also forms tumors in the cheek pouch of cortisone treated hamsters |
Products | Melanin |
Mutational profile | V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot) |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 1 x 10^5 cells/mL |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile | Amelogenin: x,y CSF1PO: 12,13 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 12 TH01: 6 TPOX: 11 vWA: 16,17 D3S1358: 14,16 D21S11: 29,32.2 D18S51: 13,16 Penta E: 7,21 Penta D: 11,13 D8S1179: 13,16 FGA: 18,20 |
HLA alleles | A*: 26:01:01 B*: 35:01:01, 38:01:01 C*: 04:01:01, 12:03:01 DRB1*: 04:02:01 DQA1*: 03:01:01 DQB1*: 03:02:01 DPB1*: 04:01:01 E: 01:01:01, 01:03:01 |