SK-MEL-2
Marque : CLS Cell Lines Service
SK-MEL-2 Cells
General information
Description | Introducing SK-MEL-2 cells, a cell line derived from a 60-year-old, White, male patient with malignant melanoma. These cells express wildtype B-Raf and mutant N-Ras (Q61R). With a doubling time of 32 hours, SK-MEL-2 cells provide a valuable tool for studying melanoma. Melanoma arises when mutations occur in melanocytes, leading to uncontrolled multiplication and cancer development. By using SK-MEL-2 cells, researchers can gain insights into melanoma's mechanisms and explore potential treatments. |
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Organism | Human |
Tissue | Skin |
Disease | Melanoma |
Metastatic site | Skin of thigh |
Synonyms | SK-Mel-2, SK-Mel 2, SK-mel-2, SK-MEL2, SK.MEL.2, SK Mel 2, SK MEL 2, SKMEL-2, SKMEL2, SKmel2, SK-ML2, SKml2 |
Characteristics
Age | 60 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Polygonal |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SK-MEL-2 (Cytion catalog number 300423) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B |
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Tumorigenic | Yes, in nude mice. Forms malignant melanoma |
Karyotype | (P6) hypodiploid to hypertetraploid with abnormalities including dicentrics, secondary constrictions and large telocentric marker. Phenotype Frequency Product: 0.0742 |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 10,12 D13S317: 11 D16S539: 8,9 D5S818: 12,13 D7S820: 11,12 TH01: 9 TPOX: 8,9 vWA: 17,18 D3S1358: 14,16 D21S11: 29,30 D18S51: 15,16 Penta E: 7,16 Penta D: 10,15 D8S1179: 12,13 FGA: 19,21,25 |