SK-UT-1B growing culture
Référence 330406
Conditionnement : 1cellcultureflask
Marque : CLS Cell Lines Service
SK-UT-1B Cells
General information
Description | This is a sub strain of SK-UT-1 which reportedly differs in morphology, karyology and in the type of tumor which develops on animal inoculation. |
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Organism | Human |
Tissue | Uterine |
Disease | Mixed mesodermal tumor. Consistent with leiomyosarcoma (grade III) |
Synonyms | SKUT1B, SK-UT 1B, SKUT-1B, Skut1B |
Characteristics
Age | 75 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SK-UT-1B (Cytion catalog number 300406) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | Me-2, 1-2, PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0590 |
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Tumorigenic | Yes, in nude mice. Forms well differentiated adenocarcinoma consistent with endometrial carcinoma |
Karyotype | (P27) hypodiploid to diploid |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:5 to 1:10 is recommended |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | CSF1PO: 10,11 D13S317: 10,13 D16S539: 12,14 D5S818: 10,11 D7S820: 9,1 TH01: 7 TPOX: 8 vWA: 16 D3S1358: 15,16 D21S11: 29,32.2 D18S51: 11,17 Penta E: 17 Penta D: 11,14 D8S1179: 14,15 FGA: 22,23 |
HLA alleles | A*: 0,76875, 03:01:01 B*: 44:02:01, 55:01:01 C*: 01:02:01, 05:01:01 DRB1*: 01:01:01, 04:01:01 DQA1*: 01:01:01, 03:01:01 DQB1*: 03:02:01, 05:01:01 DPB1*: 04:01:01, 04:02:01 E: 01:01:01, 01:03:02 |