Surfactant Protein A (SP-A) (APC)

There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38kD. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs.||Applications: |Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.||Recommended Dilutions:|Immunohistochemistry: paraffin|Western Blot: 0.5ug/ml. Detected SP-A in 10ug of human lung tissue lysate, band at ~35kD.|Optimal dilutions to be determined by the researcher.||Recommended Secondary Antibodies:|I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb|I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb|I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb|I1904-43: IgG, X-Adsorbed (Biotin) Pab Gt x Rb|I1904-40E: IgG, H&L (Biotin) Pab Gt x Rb|I1904-46P: IgG, H&L (Biotin) Pab Gt x Rb|I1904-42T: IgG, H&L (Biotin) Pab Gt x Rb|I1904-42V: IgG, H&L (Biotin) Pab Gt x Rb||Recommended Control: |Human lung tissue lysate||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.