B6055-2g | TCEP hydrochloride [51805-45-9] Clinisciences

Tris (2-carboxyethyl) phosphine hydrochloride (TCEP HCL) is a water soluble strong reducing agent that cleave disulfide bonds. It is a non-thiol and non-volatile solid. It can be utizlied as catalyst for the reduction of sulfonyl chlorides, N-oxides, sulfoxides and azides.

In measuring DHA (Dehydroascorbic acid) content in multiple tissues and plasma of 6-weeks-old mice, 35 mM TCEP HCL fully reduced DHA to AA (Ascorbic acid) after 2 hours on ice in a 5% solution of metaphosphoric acid containing 1 mM ethylenediaminetetraacetic acid (EDTA) at pH 1.5 [1]. In Aspergillus saitoi protease type XIII and porcine pepsin, TCEP can also be used in proteolysis and hydrogen/deuterium exchange [2].

Reference:
1. Sato Y, Uchiki T, Iwama M, Kishimoto Y et al. Determination of dehydroascorbic acid in mouse tissues and plasma by using tris(2-carboxyethyl)phosphine hydrochloride as reductant in metaphosphoric acid/ethylenediaminetetraacetic acid solution. Biol Pharm Bull. 2010;33(3):364-9.
2. Zhang HM, McLoughlin SM, Frausto SD et al. Simultaneous reduction and digestion of proteins with disulfide bonds for hydrogen/deuterium exchange monitored by mass spectrometry. Anal Chem. 2010 Feb 15;82(4):1450-4.

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	286.65
Cas No.	51805-45-9
Formula	C₉H₁₆ClO₆P
Solubility	≥28.7 mg/mL in H2O; insoluble in EtOH; ≥25.7 mg/mL in DMSO
Chemical Name	3,3',3''-phosphinetriyltripropanoic acid hydrochloride
SDF	Download SDF
Canonical SMILES	O=C(O)CCP(CCC(O)=O)CCC(O)=O.Cl
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Purity = 98.00%

TCEP hydrochloride [51805-45-9]

Référence B6055-2g

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