Disease:

Acute monocytic leukemia

Origin:

This cell line was established from the peripheral blood of a 1 year old human male with acute monocytic leukemia.

Species:

Homo sapiens, human

Tissue:

Peripheral blood

Morphology:

Monocyte

Properties:

Suspension

Cytogenic data:

Not specified

Patient:

Male, 1 years of age infant

Medium:

AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS + 2-mercaptoethanol (0.05 mM)

Subculture:

Cultures can be maintained by the addition or replacement of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 x 10^5 viable cells/mL. Subculture when cell concentration reaches 8x10^5 cells/mL. Do not allow the cell concentration to exceed 1 x 10^6 cells/mL. Culture at 5% CO2, 37°C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO