Troponin T, Cardiac (cTnT) (FITC)

Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I and to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure.||Applications: |Suitable for use in ELISA, Immunoaffinity Purification and Western Blot. Other applications have not been tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.|||Hybridoma:|Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.