VEGF Receptor 1 (FLT1) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommended Dilution	Western Blot: This VEGFR1 antibody can be used at 0.5-2.0 µg/ml with the appropriate secondary reagents to detect native Human sVEGFR-1/Flt-1. Immunofluorescence: 1-5 µg/ml.
Reactivities	Human
Host	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	Peptide of the unique C-terminal end of native Human soluble VEGFR-1/Flt-1
Specificity	This antibody recognizes the recombinant soluble VEGFR-1/FLT-1 (native) but not the recombinant soluble VEGFR-1/FLT-1 (D1-5) both produced in Insects cells. Because of the different glycosylation of insect cells compared to mammalian cells the MW of sFlt-1 (native) is about 96 kDa instead of about 110 kDa of the naturally occurring sFlt-1. The antibody did not cross react with recombinant Human soluble KDR (D1-7).
Formulation	PBS, pH 7.2 without preservatives or stabilizers State: Azide Free State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	fms related tyrosine kinase 1
Database Link	Entrez Gene 2321 Human UniProt ID P17948
Background	Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulinlike sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors, including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
Synonyms	VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1
Reference Data