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Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green, and mouse mAb to Actin dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites
Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green, and mouse mAb to Actin dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites
Western blot analysis of whole cell lysates using rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa
Western blot analysis of whole cell lysates using rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa