Zinc BioAssay™ Kit

Sample Type:|Serum, plasma, urine.||Intended Use:|This kit can be used for in vitro quantitative determination of zinc ion (Zn2+) in serum, plasma or urine.||Sensitivity: |The actual limite of detection (LOD) is no more than 3.0umol/L.||Range: |0~61.2umol/L, r2≥0.995||Test Principle:|The Zn2+ in the sample can combine with 5-Br-PAPS in the reagent to form a colored complex at room temperature. The color of the complex is in directly proportional to the concentration of Zn2+ in the sample within the range of 400ug/dL.||Accuracy: |Relative deviation≤15.0%||Precision: |Intra-Assay CV: <4%|Inter-Assay CV: <6%||Kit Components (see Protocol for final kit quantities):|Reagent 1,|Ascorbic acid, 50mmol/L|4- (2-hydroxyethyl) -1-piperazine-ethanesulfonic acid (Hepes) buffer (pH 6.0), 200mmol/L|Trisodium citrate (reaction enhancing solution), 0.2mol/L||Reagent 2,|2-(5-Bromo-2-pyridylazo)-5-[N-n-propyl-N-(3- sulfopropyl)|Amino phenol, disodium salt, dihydrate (5-Br- PAPS), 20umol/L||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.