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> α-Amylase Activity Assay Kit

α-Amylase Activity Assay Kit

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Referência E-BC-K007-M-96T

Tamanho : 96T

Marca : Elabscience

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Telefone : +1 850 650 7790

Detection Principle The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance, which is inactivated by the thermolabile nature of β-amylase, and then the enzyme activity of α-amylase is determined.
Sample Type Animal tissue,Plant tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (535-540 nm,optimum wavelength: 540 nm)
Research Area Plant Physiology Series
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.0097 U/mL
Detection Range 0.0097-0.3474 U/mL
Precision inter-assay CV: 3.9% | intra-assay CV: 0.03
Assay Time 40 min
Cat.No. Product Name Sizes
E-BC-K005-M β-Amylase Activity Assay Kit 96T
E-BC-K006-M α-Amylase and β-Amylase Activity Assay Kit 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
1% Epipremnum aureum tissue homogenate 1
1% Green pepper tissue homogenate 1
1% Corn grain tissue homogenate 1
1% Daucus carota tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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