Acetylcholine Assay Kit, BioAssay™

Acetylcholine Assay Kit provides a simple, direct and high throughput assay for measuring acetylcholine in biological samples. ||Principle of the Assay:|In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585nm) is directly proportional to the acetylcholine concentration in the sample.||Applications:|Assays: Determination of acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue and cell culture.|Drug Discovery/Pharmacology: Investigation of effects of drugs on acetylcholine metabolism.||Sample Size: |20ul||Linear Detection Range: |100-200uM (fluorometric assay); 0.4-10uM (colorimetric assay)||Kit Components:|A0526-32A1: ACHE Enzyme, 1x120ul|A0526-32A2: Enzyme Mix, 1x120ul|A0526-32A3: Acetylcholine Standard (2mM), 1x400ul|A0526-32A4: Dye Reagent, 1x120ul|A0526-32A5: Assay Buffer, 1x10ml||Storage and Stability:|Store all components at -20°C. Kit is stable for up to 3 months upon receipt.