Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

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Referência E-BC-K235-M-96T

Tamanho : 96T

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : Elabscience

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

Detection Principle	ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Synonyms	ALT;GPT;Glutamic-pyruvic transaminase
Sample Type	Serum,Plasma,Animal tissue,Cell,Cell culture supernatant
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (500-520 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.75 IU/L
Detection Range	0.75-72.30 IU/L
Precision	inter-assay CV: 0.09 \| intra-assay CV: 5.3%
Assay Time	1 h 20 min

Cat.No.	Product Name	Sizes
E-BC-K236-M	Aspartate Aminotransferase (AST/GOT) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Human plasma	1
Porcine serum	1
Rat serum	1
10% Rat brain tissue homogenization	1
10% Rat heart tissue homogenization	1
10% Rat liver tissue homogenization	40-60
10% Rat kidney tissue homogenization	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.