Aldehyde Dehydrogenase (ALDH) Activity Assay Kit
Referência E-BC-K565-M-96T
Tamanho : 96T
Marca : Elabscience
Detection Principle | The detection principle of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD+ into NADH, which under the action of electron coupling agent, transfer electrons to WST-8 to produce the yellow product. The activity of ADH can be calculated by measuring the change of absorbance value at 450 nm. |
Synonyms | ALDH |
Sample Type | Serum,Plasma,Animal tissue |
Detection Method | Colorimetric method |
Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
Research Area | Liver And Renal Function |
Storage | This product can be stored at -20°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.01 U/L |
Detection Range | 0.01-10.0 U/L |
Precision | inter-assay CV: 7.3% | intra-assay CV: 0.03 |
Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
10% Rat kidney tissue homogenate | 4-8 |
10% Mouse kidney tissue homogenate | 4-8 |
10% Mouse liver tissue homogenate | 4-8 |
10% Mouse lung tissue homogenate | 4-8 |
Mouse serum | 4-8 |
Rat serum | 4-8 |
Human serum | 4-8 |
Rat plasma | 4-8 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
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Elab Fluor®405
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