Anti-APC antibody (2600-2760)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	APC
Gene ID:	324
Uniprot ID:	APC_HUMAN
Immunogen Region:	2600-2760
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2600-2760 of human APC (NP_000029.2).
Immunogen Sequence:	QSKENQVSAKGTWRKIKENE FSPTNSTSQTVSSGATNGAE SKTLIYQMAPAVSKTEDVWV RIEDCPINNPRSGRSPTGNT PPVIDSVSEKANPNIKDSKD NQAKQNVGNGSVPMRTVGLE NRLNSFIQVDAPDQKGTEIK PGQNNPVPVSETNESSIVER T

Tissue Specificity	Expressed in a variety of tissues: brain, small intestine, colon, thymus, skeletal muscle, heart, prostate, lung, spleen, ovary, testis kidney, placenta, blood and liver. Isoform 1A: Very strongly expressed in brain but has relatively low expression levels in other tissues. Isoform 1B: Predominant form in all tissues except for brain, including gastric mucosa and blood.
Post Translational Modifications	Phosphorylated.phosphorylation enhances the F-actin bundling activity. Phosphorylated by GSK3B. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is facilitated by Axin. Deubiquitinated by ZRANB1/TRABID.
Function	Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Associates with both microtubules and actin filaments, components of the cytoskeleton. Plays a role in mediating the organization of F-actin into ordered bundles. Functions downstream of Rho GTPases and DIAPH1 to selectively stabilize microtubules. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
Protein Name	Adenomatous Polyposis Coli Protein Protein Apc Deleted In Polyposis 2.5
Database Links	Reactome: R-HSA-111465 Reactome: R-HSA-195253 Reactome: R-HSA-196299 Reactome: R-HSA-3769402 Reactome: R-HSA-4641262 Reactome: R-HSA-5339716 Reactome: R-HSA-5358747 Reactome: R-HSA-5358749 Reactome: R-HSA-5358751 Reactome: R-HSA-5358752 Reactome: R-HSA-5467333 Reactome: R-HSA-5467337 Reactome: R-HSA-5467340 Reactome: R-HSA-5467348 Reactome: R-HSA-5689896
Cellular Localisation	Cell Junction Adherens Junction Cytoplasm Cytoskeleton Cell Projection Lamellipodium Ruffle Membrane Cell Membrane Associated With The Microtubule Network At The Growing Distal Tip Of Microtubules Mapre1 May Be Required For Targeting To The Growing Microtubule Plus Ends Accumulates In The Lamellipodium And Ruffle Membrane In Response To Hepatocyte Growth Factor (Hgf) Treatment The Memo1-Rhoa-Diaph1 Signaling Pathway Controls Localization Of The Phosphorylated Form To The Cell Membrane
Alternative Antibody Names	Anti-Adenomatous Polyposis Coli Protein antibody Anti-Protein Apc antibody Anti-Deleted In Polyposis 2.5 antibody Anti-APC antibody Anti-DP2.5 antibody

Information sourced from Uniprot.org

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