Anti-Cenexin-1 (RABBIT) Antibody
Referência 600-401-A46
Tamanho : 100ug
Marca : Rockland Immunochemicals
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Specifications for Cenexin-1 Antibody
Product Details
Anti-Cenexin-1 (RABBIT) Antibody - 600-401-A46
rabbit anti-Cenenxin1 antibody, anti-Cenenxin-1, anti-Cenenxin 1, ODF84 antibody, Outer dense fiber of sperm tail 2, Cenexin1 variant 1 antibody, KKT 4 antibody, Outer dense fiber protein 2, ODF2
Rabbit
Polyclonal
IgG
Target Details
ODF2 - View All ODF2 Products
Human, Chimpanzee, Macaque Monkey
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the carboxy terminus.
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using peptide coupled to agarose beads. This antibody is specific for human Cenexin-1 protein. A BLAST analysis was used to suggest cross-reactivity with Cenexin-1 from human, chimpanzee and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with Cenexin-1from other sources has not been determined.
Q5BJF6 - UniProtKB
NP_702915 - NCBI Protein
4957 - Gene ID
Application Details
ELISA, WB
IF - View References
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 93 kDa in size corresponding to Cenexin-1 by western blotting in the appropriate cell lysate or extract.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cenexin-1, also known as ODF2 and outer dense fiber of sperm tails 2, are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.
References (1)
Gaume et al. (2015). Centrosomal nucleolin is required for microtubule network organization. Cell Cycle
Applications
IF, Confocal Microscopy PubMed
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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