Anti-N-ACYLMANNOSAMINE-1-DEHYDROGENASE (Pseudomonas) (GOAT) Antibody
Referência 200-101-228S
Tamanho : 25ul
Marca : Rockland Immunochemicals
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Telefone : +1 850 650 7790
Specifications for N-Acylmannosamine-1-Dehydrogenase Antibody
Product Details
Anti-N-Acylmannosamine-1-Dehydrogenase (Pseudomonas) (GOAT) Antibody (BULK ORDER) - 200-101-228
goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody, N-acylmannosamine 1-dehydrogenase, NAM-DH
Goat
Polyclonal
IgG
Target Details
NAM-DH - View All NAM-DH Products
Bacteria
Native Protein
N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
Anti-N-ACYLMANNOSAMINE-1-DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified N-Acylmannosamine-1-Dehydrogenase [Pseudomonas]. Cross reactivity against N-Acylmannosamine-1-Dehydrogenase from other sources is unknown.
P22441 - UniProtKB
P22441.3 - NCBI Protein
7614553 - Gene ID
Application Details
WB
N-ACYLMANNOSAMINE-1-DEHYDROGENASE Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of N-Acylmannosamine-1-Dehydrogenase [Pseudomonas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
5.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
N-acylmannosamine 1-dehydrogenase acts on acetyl-D-mannosamine and glycolyl-D-mannosamine. NAM-DH may be applicable to the quantitative determination of sialic acid in serum.
Related Protocols
Adherent Cell Lysis Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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