Goat Polyclonal

Application / Dilution
ICC1:200
IHC1:200

Size 0.5 ml

Species Reactivity Rt

DyLight® fluorophores have absorption maxima covering the entire visible light spectrum (350 nm to 777 nm), as well several key near-infrared and infrared wavelengths. Both the absorption and emission properties of the DyLight® fluorophores match the excitation and detection wavelengths of common fluorescence instrumentation used for immunocytochemistry, immunohistochemistry, and flow cytometry. The DyLight® fluorophores exhibit higher fluorescence intensity and photostability than Alexa Fluor, CyDye and LI-COR Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9). Additionally, the water solubility of the DyLight® fluorophores allows a high dye-to-protein ratio to be achieved without causing precipitation of conjugates. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make these fluorophores attractive as fluorescent probes in a variety of fluorescence methods.

 

References
Alkhatatbeh MJ (2011) J Thromb Haemost. 9(4):844.
Sarkar P. et al. (2010) J Photochem Photobiol B. 98(1):35.

Immunocytochemical labeling of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-α-Tubulin (TM4111) (left) and rat monoclonal anti-CLASP2 (CM5051) (middle). The antibodies were detected using either goat anti-mouse:DyLight® 488 (MS3011) or goat anti-Rat:DyLight® 594 (RS3111).



The DyLight® 594-conjugated goat polyclonal secondary reagent reacts with the light chains of rat IgG, and the light chains of other rat immunoglobulins. The secondary reagent has minimal cross-reactivity with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins.

Goat polyclonal antibody is supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This antibody has been pre-adsorbed with various immunoglobulins from non-rat species before affinity-purification using rat IgG light chain coupled to agarose beads. Purified goat polyclonal antibody was conjugated to DyLight® 594 fluorophore (Excitation = 593 nm; Emission = 618 nm). This secondary reagent can be used to detect rat immunoglobulin light chains in various immunofluorescence applications, such as immunocytochemistry, immunohistochemistry, and flow cytometry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: