MAB6762 | ATF6 monoclonal antibody, clone 1-7 Clinisciences

Specifications

Product Description

Mouse monoclonal antibody raised against partial recombinant ATF6.MAB,monoclonal Ab,monoclonal antibody

Immunogen

Recombinant His fusion protein corresponding to N-terminus human ATF6.

Host

Mouse

Reactivity

Human

Form

Liquid

Isotype

IgG2a, kappa

Recommend Usage

The optimal working dilution should be determined by the end user.

Storage Buffer

In PBS (50% glycerol)

Storage Instruction

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Applications

Western Blot (Cell lysate)

Western blot analysis of human cell extracts using ATF6 monoclonal antibody, clone 1-7 (Cat # MAB6762).
Conversion of pATF6alpha (P) to pATF6alpha (N) in DTT- or tunicamycin-treated cells. Tm: 2 ug/mL tunicamycin (inhibitor of N-glycosylation). DTT : 1mM dithiothreitol (reducing reagent). The asterisk denotes an unglycosylated form of pATF6alpha (P).
ATF6alpha is constitutively expressed as pATF6alpha (P) (~90-kDa protein), and converted to pATF6alpha (N) (>50-kDa protein) in ER-stressed cells.

Immunoprecipitation

IP-Western blot analysis of human cell extracts using ATF6 monoclonal antibody, clone 1-7 (Cat # MAB6762).

Gene Info

Entrez GeneID

22926

Protein Accession#

P18850

Gene Name

ATF6

Gene Alias

ATF6A

Gene Description

activating transcription factor 6

Omim ID

605537

Gene Ontology

Hyperlink

Gene Summary

ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.[supplied by OMIM

Other Designations

OTTHUMP00000025585|activating transcription factor 6 alpha

Interactomes

ATF6

Diseases

Publication Reference

The p38-activated ER stress-ATF6α axis mediates cellular senescence.
Kim HS, Kim Y, Lim MJ, Park YG, Park SI, Sohn J.

FASEB Journal 2018 Sep; fj201800836R.

Application：WB-Tr, Human, MCF-7 cells.
Identification and characterization of two novel spliced genes located in the orf47-orf46-orf45 gene locus of Kaposi's sarcoma-associated herpesvirus.
Chang PJ, Hung CH, Wang SS, Tsai PH, Shih YJ, Chen LY, Huang HY, Wei LH, Yen JB, Lin CL, Chen LW.

Journal of Virology 2014 Sep; 88(17):10092.

Application：WB-Tr, Human, 293T cells.
Transcriptional induction of mammalian ER quality control proteins is mediated by single or combined action of ATF6alpha and XBP1.
Yamamoto K, Sato T, Matsui T, Sato M, Okada T, Yoshida H, Harada A, Mori K.

Developmental Cell 2007 Sep; 13(3):365.

Application：IP, WB, Human, HeLa cells.
Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress.
Haze K, Yoshida H, Yanagi H, Yura T, Mori K.

Molecular Biology of the Cell 1999 Nov; 10(11):3787.
Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.
Hai TW, Liu F, Coukos WJ, Green MR.

Genes & Development 1989 Dec; 3(12B):2083.

ATF6 monoclonal antibody, clone 1-7

Referência MAB6762

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Marca : Abnova