Application
| WB, ICC |
|---|---|
| Primary Accession | O75143 |
| Other Accession | NP_001136145.1 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Description | Rabbit Anti-Human ATG13 Polyclonal |
| Target/Specificity | Detects ~56-63kDa. |
| Other Names | ATG13_Human Antibody, Autophagy related protein 13 Antibody, KIAA0652 Antibody |
| Immunogen | Synthetic peptide from the mid-protein range of human ATG13 |
| Purification | Peptide Affinity Purified |
| Storage | -20ºC |
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:1000 dilution of SPC-657 was sufficient for detection of ATG13 on HEK293 lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasm | Cytosol | Preautophagosomal structure |
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Provided below are standard protocols that you may find useful for product applications.
Background
ATG13 is an autophagy factor required for phagosome formation. It is a target of the TOR kinase signaling pathway that regulates autophagy through phosphorylation of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (1-3).
References
1. Mercer C.A., Kaliappan A., Dennis P.B. (2009) Autophagy. 5(5): 649-662.
2. Hosokawa N., et al. (2009) Mol Biol Cell. 20(7): 1981-1991.
3. Chan E.Y., Longatti A., McKnight N.C., Tooze S.A. (2009) Mol Cell Biol. 29(1): 157-171.