Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

Biotin [58-85-5]

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Referência HY-B0511-1g

Tamanho : 1g

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Biotin, vitamin B7 and serves as a coenzyme for five carboxylases in humans, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids.

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Biotin Chemical Structure

CAS No. : 58-85-5

This product is a controlled substance and not for sale in your territory.

Based on 11 publication(s) in Google Scholar

Other Forms of Biotin:

Biotin-d₂-1 Obtenir un devis
rel-Biotin-d₄ Obtenir un devis
Biotin-d₂ Obtenir un devis
Biotin sodium Obtenir un devis
Biotin (Standard) Obtenir un devis

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

In Vitro

Biotin exhibits higher affinity on breast cancer (T47D) cells over normal mammary epithelial (MCF-12A) cells, with K_ms of 9.24 μM and 53.1 μM, respecetively^[4].
Biotin (0.09-100 μM; 0-70 min) is dose-dependently uptake by T47D cells with V_max of 27.34 pmol/mg protein/min^[4].
Biotin (1-1000 nM; 24 h) exerts function in the recovery of 7β-OHC (50 µM)-induced cell death and reduces cell adhesion^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[5]

Cell Line:	Murine oligodendrocyte 158N cells
Concentration:	1, 10, 100, 1000 nM
Incubation Time:	24 hours
Result:	Showed cytoprotective effects and prevents 7β-hydroxycholesterol-induced disruption of redox status. Improved attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration.

In Vivo

Biotin (15 mg/kg/d; p.o.; 12 d) improves nephrotoxicity in streptozotocin-induced (150 mg/kg; i.p.) diabetic mice^[6].
Biotin (0.012 mg/kg/d; p.o.; 70 d) deficiency impaires the immune function of the head kidney, spleen and skin in fish with inadequate administration dose^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Streptozotocin-induced Diabetes type 1 in male Swiss albino mice (25±2 g)^[6]
Dosage:	15 mg/kg/d
Administration:	Oral gavage; 12 days
Result:	Improved histopathological results, including distorted glomeruli, inflammatory cells, and giant macrophages, and diminished acroline reaction of oxidative damage.

Animal Model:	Grass carp (117±0.5 g)^[7]
Dosage:	0.012, 0.110, 0.214, 0.311, 0.427, and 0.518 mg/kg
Administration:	Oral gavage; 70 days
Result:	Reduced the activities of lysozyme (LZ) and acid phosphatase (ACP), decreased the contents of complement 3 (C3), C4 and immunoglobulin M (IgM). Reduced the mRNA levels of anti-microbial substances. Increased the mRNA levels of pro-inflammatory cytokines, tumour necrosis factor partially and reduced anti-inflammatory IL-4/13A, IL-10, IL-11 and TGF-β1 mRNA levels partially in association with target of rapamycin (TOR) signaling.

Essai clinique

Masse moléculaire

244.31

Formule

C₁₀H₁₆N₂O₃S

CAS No.

58-85-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CCCC[C@@H]1SC[C@]([C@]1(04)N2)(04)NC2=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite
Disease markers
Immune System Disorder

Plants
other families

Microorganisms

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (409.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0932 mL	20.4658 mL	40.9316 mL
5 mM	0.8186 mL	4.0932 mL	8.1863 mL
10 mM	0.4093 mL	2.0466 mL	4.0932 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 1.96 mg/mL (8.02 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.80%

Select Batch:

SDS (394 KB)

COA (251 KB) HNMR (206 KB) LCMS (386 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Zempleni, et al. Biotin biochemistry and human requirements. J Nutr Biochem, 1999. 10(3): p. 128-38. [Content Brief]

[2]. Mock, D.M.. Biotin status: which are valid indicators and how do we know? J Nutr, 1999. 129(2S Suppl): p. 498S-503S. [Content Brief]

[3]. Zempleni J, et al. Biotin. Biofactors. 2009 Jan-Feb;35(1):36-46. [Content Brief]

[4]. Vadlapudi AD, et al. Biotin uptake by T47D breast cancer cells: functional and molecular evidence of sodium-dependent multivitamin transporter (SMVT). Int J Pharm. 2013 Jan 30;441(1-2):535-43. [Content Brief]

[5]. Sghaier R, et al. Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes. Free Radic Res. 2019 May;53(5):535-561. [Content Brief]

[6]. Aldahmash BA, et al. Biotin amelioration of nephrotoxicity in streptozotocin-induced diabetic mice. Saudi J Biol Sci. 2015 Sep;22(5):564-9. [Content Brief]

[7]. He P, et al. Dietary biotin deficiency decreased growth performance and impaired the immune function of the head kidney, spleen and skin in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2020 Feb;97:216-234. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0932 mL	20.4658 mL	40.9316 mL	102.3290 mL
	5 mM	0.8186 mL	4.0932 mL	8.1863 mL	20.4658 mL
	10 mM	0.4093 mL	2.0466 mL	4.0932 mL	10.2329 mL
	15 mM	0.2729 mL	1.3644 mL	2.7288 mL	6.8219 mL
	20 mM	0.2047 mL	1.0233 mL	2.0466 mL	5.1165 mL
	25 mM	0.1637 mL	0.8186 mL	1.6373 mL	4.0932 mL
	30 mM	0.1364 mL	0.6822 mL	1.3644 mL	3.4110 mL
	40 mM	0.1023 mL	0.5116 mL	1.0233 mL	2.5582 mL
	50 mM	0.0819 mL	0.4093 mL	0.8186 mL	2.0466 mL
	60 mM	0.0682 mL	0.3411 mL	0.6822 mL	1.7055 mL
	80 mM	0.0512 mL	0.2558 mL	0.5116 mL	1.2791 mL
	100 mM	0.0409 mL	0.2047 mL	0.4093 mL	1.0233 mL

Biotin Related Classifications

Metabolic Disease

Metabolic Enzyme/Protease
Endogenous Metabolite

Biotin [58-85-5]

Referência HY-B0511-1g

Contactar o distribuidor local :

Marca : MedChemExpress

Contactar o distribuidor local :

Other Forms of Biotin:

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Biotin Related Classifications

Keywords:

Você também pode estar interessado nos seguintes produtos:

Biotin [58-85-5]

Referência HY-B0511-1g

Contactar o distribuidor local :

Marca : MedChemExpress

Contactar o distribuidor local :

Other Forms of Biotin:

Biotin Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Biotin Related Classifications

Keywords:

Você também pode estar interessado nos seguintes produtos: