Product Details
Bovine Serum Albumin - Fraction V (Immunoglobulin and Protease Free) - BSA-10
Bovine serum albumin fraction V, Bovine Albumin, BSA, BSA Blocker, BSA Blocking
Bovine
Target Details
Purity (%): 100% by Agarose Zone electrophoresis Moisture (%) 5% Loss on Drying pH: 7.0 Ash: <2.0% Protein (%): 98% by nitrogen analysis Protease: <0.005 Units/mg Heavy Metals: (Pb) < 10 ppm by ICP Endotoxin: < 3 EU/mg by LAL IgG: Not Detected NEFA: N/A
Application Details
ELISA, FC, Functional Assay, IF, IHC, WB, LFA - View References
Bovine Serum Albumin - Fraction V is suitable for use in lateral flow, in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding for FACS, IF, IHC, and WB.
Formulation
None
None
None
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store container at 4° C prior to opening.
Expiration date is one (1) year from date of receipt.
Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
Afshar-Saber W et al. (2024). ALDH5A1-deficient iPSC-derived excitatory and inhibitory neurons display cell type specific alterations. Neurobiol Dis.
Applications
Blocking PubMed
Helble JD et al. (2023). Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection. iScience.
Applications
Buffer PubMed
Chen W et al. (2023). The mRNA-binding protein DDX3 mediates TGF-β1 upregulation of translation and promotes pulmonary fibrosis. JCI Insight.
Applications
IHC, ICC, Histology PubMed
Neira V et al. (2023). Identification and characterization of porcine Rotavirus A in Chilean swine population. Front Vet Sci.
Applications
Buffer PubMed
Christopher Yanucil et al. (2022). Soluble α-klotho and heparin modulate the pathologic cardiac actions of fibroblast growth factor 23 in chronic kidney disease. Kidney Int.
Applications
IF, Confocal Microscopy; IHC, ICC, Histology PubMed
Gargaro, M et al. (2022). Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication. Immunity
Applications
Buffer; Blocking PubMed
Yanucil, C et al. (2022). FGF21-FGFR4 signaling in cardiac myocytes promotes concentric cardiac hypertrophy in mouse models of diabetes. Scientific Reports
Applications
Blocking PubMed
Chio TI et al. (2022). A BODIPY-Based Probe Enables Fluorogenicity via Thiol-Dependent Modulation of Fluorophore Aggregation. Molecules.
Applications
SDS, 2D-PAGE PubMed
Pilling D et al. (2022). The sialidase NEU3 promotes pulmonary fibrosis in mice. Respir Res.
Applications
IHC, ICC, Histology PubMed
Lee IK et al. (2022). Monitoring Therapeutic Response to Anti-FAP CAR T Cells Using [18F]AlF-FAPI-74. Clin Cancer Res.
Applications
IF, Confocal Microscopy PubMed
Kirolos SA et al. (2022). The extracellular sialidase NEU3 primes neutrophils. J Leukoc Biol.
Applications
Hemagglutination Inhibition (HI) Assay PubMed
Kurosaki T et al. (2022). Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells. Mol Cell.
Applications
IF, Confocal Microscopy PubMed
Cho H et al. (2022). AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay. Mol Cell.
Applications
Blocking PubMed
Ahn TS et al. (2021). Commercial immunoglobulin products contain cross-reactive but not neutralizing antibodies against SARS-CoV-2. J Allergy Clin Immunol.
Applications
E, EIA PubMed
Boccasavia, VL et al. (2021). Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen. Cell Reports
Applications
FC, FACS, FLOW; Buffer PubMed
Kurosaki, T et al. (2021). Loss of the fragile X syndrome protein FMRP results in misregulation of nonsense-mediated mRNA decay. Nature Cell Biology
Applications
Control; Blocking PubMed
Rahman F et al. (2021). DYNC1LI2 regulates localization of the chaperone-mediated autophagy receptor LAMP2A and improves cellular homeostasis in cystinosis. Autophagy.
Applications
IHC, ICC, Histology PubMed
Papimi D et al. (2021). The Aurora B gradient sustains kinetochore stability in anaphase. Cell Rep.
Applications
IF, Confocal Microscopy PubMed
Woodworth MA et al. (2021). Multiplexed single-cell profiling of chromatin states at genomic loci by expansion microscopy. Nucleic Acids Research
Applications
IF, Confocal Microscopy PubMed
Portillo AL et al. (2021). Production of human CAR-NK cells with lentiviral vectors and functional assessment in vitro. STAR Protoc.
Applications
FC, FACS, FLOW PubMed
Burgos-Bravo F et al. (2020). Application of Force to a Syndecan-4 Containing Complex With Thy-1–αVβ3 Integrin Accelerates Neurite Retraction. Front Mol Biosci.
Applications
EIA, ELISA PubMed
Zhang J, et al. (2020). Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein. Sci Rep.
Applications
WB, IB, PCA PubMed
Zhang J, et al. (2020). Blockade of Macrophage Adhesion to CD200-treated Polystyrene Culture Surface. J Biomed Mater Res A.
Applications
WB, IB, PCA PubMed
Porterfield V. et al. (2020). A three-dimensional dementia model reveals spontaneous cell cycle re-entry and a senescence-associated secretory phenotype. Neurobiol Aging.
Applications
IF, Confocal Microscopy PubMed
Mao C. et al. (2020). Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy. Sci Adv.
Applications
IF, Confocal Microscopy PubMed
Schuster-Little N, Madera S, Whelan R. (2020). Developing a mass spectrometry-based assay for the ovarian cancer biomarker CA125 (MUC16) using suspension trapping (STrap). Anal Bioanal Chem.
Applications
Other PubMed
Gronseth, E et al. (2020). Astrocytes influence medulloblastoma phenotypes and CD133 surface expression. PloS One
Applications
Blocking PubMed
Leung CS, Douglass SM, Morselli M, et al. (2019). H3K36 Methylation and the Chromodomain Protein Eaf3 Are Required for Proper Cotranscriptional Spliceosome Assembly. Cell Rep.
Applications
Undefined PubMed
Pollock SR, Schinlever AR, Rohani A, Kashatus JA, Kashatus DF. (2019). RalA and RalB relocalization to depolarized mitochondria depends on clathrin-mediated endocytosis and facilitates TBK1 activation. PLoS One.
Applications
FC, FACS, FLOW PubMed
Zhang J, He J, Johnson JL, et al. (2019). Cross-regulation of defective endolysosome trafficking and enhanced autophagy through TFEB in UNC13D deficiency. Autophagy.
Applications
IF, Confocal Microscopy PubMed
Moreno-Olivas F et al. (2019). ZnO nanoparticles affect nutrient transport in an in vitro model of the small intestine. Food Chem Toxicol.
Applications
IHC, ICC, Histology PubMed
Allen JN, Dey A, Nissly R, et al. (2017). Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation. J Vis Exp.
Applications
Other PubMed
Chang Q, Ornatsky O, Hedley D. (2017). Staining of Frozen and Formalin-Fixed, Paraffin-Embedded Tissues with Metal-Labeled Antibodies for Imaging Mass Cytometry Analysis. Curr Protoc Cytom.
Applications
IF, Confocal Microscopy PubMed
Mondanelli G, Volpi C. (2017). Differentiation of Myeloid-derived Suppressor Cells from Murine Bone Marrow and Their Co-culture with Splenic Dendritic Cells. Bio Protoc.
Applications
Other PubMed
Spencer, KC et al. (2017). A three dimensional in vitro glial scar model to investigate the local strain effects from micromotion around neural implants. Lab on a Chip
Applications
IHC, ICC, Histology; Buffer PubMed
Domogauer JD, de Toledo SM, Azzam EI. (2016). A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication. J Vis Exp.
Applications
IF, Confocal Microscopy PubMed
Bucukovski, J et al. (2015). A Multiplex Label-Free Approach to Avian Influenza Surveillance and Serology. PloS One
Applications
Microarray, Microarray chip PubMed
Keeler, GD et al. (2015). Effects of delayed delivery of dexamethasone-21-phosphate via subcutaneous microdialysis implants on macrophage activation in rats. Acta Biomaterialia
Applications
Blocking PubMed
Keeler, GD et al. (2015). Localized delivery of dexamethasone-21-phosphate via microdialysis implants in rat induces M(GC) macrophage polarization and alters CCL2 concentrations. Acta Biomaterialia
Applications
Blocking PubMed
Toops, KA et al. (2014). A detailed three-step protocol for live imaging of intracellular traffic in polarized primary porcine RPE monolayers. Experimental Eye Research
Applications
Blocking; IF, Confocal Microscopy PubMed
Keeler, GD et al. (2014). Comparison of microdialysis sampling perfusion fluid components on the foreign body reaction in rat subcutaneous tissue. European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
Applications
Buffer PubMed
Clausen TM, Christoffersen S, Dahlbäck M, et al. (2012). Structural and functional insight into how the Plasmodium falciparum VAR2CSA protein mediates binding to chondroitin sulfate A in placental malaria. J Biol Chem.
Applications
Other PubMed
Lehtinen, J et al. (2012). Pre-targeting and direct immunotargeting of liposomal drug carriers to ovarian carcinoma. PloS One
Applications
Blocking PubMed
Andrew O Fung et al. (2012). Quantitative detection of PfHRP2 in saliva of malaria patients in the Philippines Malar J.
Applications
EIA, ELISA PubMed
Makino N, Maeda T, Oyama J, et al. (2011). Antioxidant therapy attenuates myocardial telomerase activity reduction in superoxide dismutase-deficient mice. J Mol Cell Cardiol.
Applications
IHC, ICC, Histology PubMed
Lee CH, Bose S, Van Vliet KJ, Karp JM, Karnik R. (2011). Studying cell rolling trajectories on asymmetric receptor patterns. J Vis Exp.
Applications
Other PubMed
Dahlbäck M, Jørgensen LM, Nielsen MA, et al. (2011). The chondroitin sulfate A-binding site of the VAR2CSA protein involves multiple N-terminal domains. J Biol Chem.
Applications
Other PubMed
Lee, Chia-Hua et al. (2011). Examining the lateral displacement of HL60 cells rolling on asymmetric P-selectin patterns. Langmuir : the Acs Journal of Surfaces and Colloids
Applications
Control PubMed
Moorjani S, Nielson R, Chang XA, Shear JB. (2010). Dynamic remodeling of subcellular chemical gradients using a multi-directional flow device. Lab Chip.
Applications
Other PubMed
Resende M, Ditlev SB, Nielsen MA, et al. (2009). Chondroitin sulphate A (CSA)-binding of single recombinant Duffy-binding-like domains is not restricted to Plasmodium falciparum Erythrocyte Membrane Protein 1 expressed by CSA-binding parasites. Int J Parasitol.
Applications
E, EIA PubMed
de la Maza MP, Olivares D, Hirsch S, et al. (2006). Weight increase and overweight are associated with DNA oxidative damage in skeletal muscle. Clin Nutr.
Applications
IHC, ICC, Histology PubMed
Dravid G, Hammond H, Cheng L. (2006). Culture of human embryonic stem cells on human and mouse feeder cells. Methods Mol Biol.
Applications
Other PubMed
McDonald JC et al. (2002). Prototyping of microfluidic devices in poly (dimethylsiloxane) using solid-object printing. Anal Chem.
Applications
IF, Confocal Microscopy PubMed
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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