BRD2 TR-FRET, BioAssay™ Kit (BD1+BD2, RNF3, RING3)

Kit comes in a convenient format with purified BRD2 protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.||The BRD2 (BD1+BD2) TR-FRET BioAssay™ Kit is designed to measure the inhibition of BRD2 (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD2, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.||Applications: |Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.||Kit Components:|White, Nonbinding Corning, low volume, microtiter plate, 1x plate|BRD2 (BD1+BD2), 1x60ug |BET Bromodomain Ligand, 1x50ul|Non-acetylated Ligand 1, 1x15ul |Tb donor, 1x20ul |Dye-labeled acceptor, 1x20ul |3x BRD TR-FRET Assay Buffer 1, 1x4ml ||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.