Bruton'S Tyrosine Kinase (Btk) BioAssay™ ELISA Kit (Human)

The Bruton'S Tyrosine Kinase (Btk) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Btk in human tissue homogenates, cell lysates and other biological fluids.||Detection Range:|0.156-10ng/ml||Sensitivity:|0.055ng/ml||Intra-Assay: |CV<10% ||Inter-Assay: |CV<12%||Kit Components:|*023792A: Microtiter Strips, 1x96wells, Pre-coated, ready to use|*023792B: Standard, 2x1vial |023792C: Standard Diluent, 1x20ml|*023792D: Detection Reagent A (green), 1x120ul |*023792E: Detection Reagent B (red), 1x120ul |023792F: Assay Diluent A, 1x12ml|023792G: Assay Diluent B, 1x12ml|023792H: TMB Substrate, 1x9ml|*023792J: Positive Control, 1.32ng/ml, 1x1vial (Lyophilized)|023792K: Stop Solution, 1x6ml|023792L: Wash Buffer, 30X, 1x20ml ||Precaution:|The Stop Solution (023792K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.||Storage and Stability:|Store *023792A, *023792B, *023792D, *023792E and *023792J at -20°C. After reconstitution of *023792J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Assay Procedure Summary:|1. Prepare all reagents, samples and standards.|2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C.|3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.|4. Aspirate and wash 3 times.|5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.|6. Aspirate and wash 5 times.|7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37°C.|8. Add 50ul Stop Solution. Read at 450nm immediatel