BU-1A (Bu1a, B6.1) (FITC)

Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development.||Applications:|Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: 1:20-1:80; 10ul labels 10e6 cells in 100ul|Optimal dilutions to be determined by the researcher.||Hybridoma: |SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.