C3A ELISA Kit (Rabbit)

Referência OKCD04289

Tamanho : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for C3A ELISA Kit (Rabbit) (OKCD04289)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)79-93%98-105%89-101%80-91%
EDTA Plasma (n=5)83-97%98-105%94-103%89-98%
Heparin Plasma (n=5)79-96%84-93%78-103%85-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 3a (C3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 3a (C3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 3a (C3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Component 3a (C3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)86-10194
EDTA Plasma (n=5)92-10398
Heparin Plasma (n=5)87-10193
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 3a (C3a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 3a (C3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
C3a Microplate96 Wells (12 x 8 Well strips)
C3a Lyophilized Standard2
100X C3a HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.33 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolC3
Gene Full Namecomplement C3
Alias Symbolscomplement C3 alpha chain;complement component 3.
NCBI Gene Id100009121
Protein NameComplement C3 alpha chain
Description of TargetC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Uniprot IDP12247
Protein Accession #NP_001075755.1
Nucleotide Accession #NM_001082286.1