Images
Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

CCNE1 MaxPab rabbit polyclonal antibody. Western Blot analysis of CCNE1 expression in mouse liver.

Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot analysis of CCNE1 expression in transfected 293T cell line (H00000898-T03) by CCNE1 MaxPab polyclonal antibody.

Lane 1: CCNE1 transfected lysate(47.10 KDa).
Lane 2: Non-transfected lysate.

<em>In situ</em> Proximity Ligation Assay (Cell)
Application

In situ Proximity Ligation Assay (Cell)

Proximity Ligation Analysis of protein-protein interactions between CCNE1 and CDKN1A. HeLa cells were stained with anti-CCNE1 rabbit purified polyclonal 1:1200 and anti-CDKN1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against a full-length human CCNE1 protein.MaxPab Polyclonal Antibody,MaxPab Polyclonal Antibodies,MaxPab,DNA Immune,DNA Immunization,Immune Technology

    Immunogen

    CCNE1 (NP_001229.1, 1 a.a. ~ 410 a.a) full-length human protein.

    Sequence

    MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA

    Host

    Rabbit

    Reactivity

    Human, Mouse

    Quality Control Testing

    Antibody reactive against mammalian transfected lysate.

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Tissue lysate)

    CCNE1 MaxPab rabbit polyclonal antibody. Western Blot analysis of CCNE1 expression in mouse liver.

    Western Blot (Transfected lysate)

    Western Blot analysis of CCNE1 expression in transfected 293T cell line (H00000898-T03) by CCNE1 MaxPab polyclonal antibody.

    Lane 1: CCNE1 transfected lysate(47.10 KDa).
    Lane 2: Non-transfected lysate.

    In situ Proximity Ligation Assay (Cell)

    Proximity Ligation Analysis of protein-protein interactions between CCNE1 and CDKN1A. HeLa cells were stained with anti-CCNE1 rabbit purified polyclonal 1:1200 and anti-CDKN1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Gene Info — CCNE1

    Entrez GeneID

    898

    GeneBank Accession#

    NM_001238

    Protein Accession#

    NP_001229.1

    Gene Name

    CCNE1

    Gene Alias

    CCNE

    Gene Description

    cyclin E1

    Omim ID

    123837

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq

    Other Designations

    cyclin Es|cyclin Et

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