CD16-Like (FcRIII) (PE)

Referência 227406-PE-100ul

Tamanho : 100ul

Marca : US Biological

Solicitar mais informações

Contactar o distribuidor local :


Telefone : +1 850 650 7790


227406-PE CD16-Like (FcRIII) (PE)

Clone Type
Polyclonal
Host
mouse
Source
sheep
Isotype
IgG1
Grade
Affinity Purified
Applications
E IHC IP
Crossreactivity
Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Recognizes a cell surface antigen of 65-80kD which is similar to the human cell surface antigen designated as CD16. It has therefore been hypothesized that may recognize the ovine homologue of human CD16 (Hopkins, J. et. al. 1996).||Recognizes an antigen that is strongly expressed by ovine alveolar macrophages and peripheral blood granulocytes, and more weakly by monocytes and other tissue macrophages.||Applications: |Suitable for use in Flow Cytometry (Not Tested), ELISA, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Flow Cytometry (Not Tested): 1:25-1:200|ELISA: 1:500|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: VPM64|Host: mouse|Source: sheep|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Ovine macrophages.|Specificity: Recognizes sheep CD16-Like.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Ovine macrophages.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes sheep CD16-Like.
References
1. Hopkins, J. et al. (1996) Identification of three myeloid specific differentiation antigens in sheep. Vet. Immunol. Immunopathol. 52: 329-339. 2. Caroprese, M. et al. (2008) Technical note: immunomagnetic procedure for positive selection of macrophages in ovine milk. J Dairy Sci. 91:1908-12.