CD49d (APC)

Referência 516415-500T

Tamanho : 500Tests

Marca : US Biological

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516415 CD49d (APC) discontinued

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P13612
Isotype
IgG1,k
Grade
Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD49d is a 150kD alpha integrin chain known as alpha4 integrin or VLA-4 alpha chain. It forms a heterodimer with either integrin beta1 (alpha4beta1, VLA-4) or beta7 (alpha4beta7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. alpha4beta7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: 2ul or 5ul of antibody/test (million cells in 100ul staining volume or per 100ul of whole blood).|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: BU49|Host: mouse|Source: human|Concentration: As reported |Form: Supplied as a liquid in PBS, 0.05% Proclin300, 1% BSA. Labeled with Allophycocyanin (APC).|Purity: Purified|Specificity: Recognizes human CD49d.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, 0.05% Proclin300, 1% BSA. Labeled with Allophycocyanin (APC).
Purity
Purified
Specificity
Recognizes human CD49d.
References
1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4).|2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722.