CD79A (208-222) Mouse Monoclonal Antibody [Clone ID: HM47]

Specifications

Product Data
Clone Name	HM47
Applications	FC
Recommended Dilution	Flow cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivities	Bovine, Canine, Chicken, Equine, Guinea Pig, Human, Mouse, Porcine, Primate, Rabbit, Rat
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
Specificity	This antibody reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
Formulation	Phosphate buffered saline (PBS) Label: PE State: Liquid Ig fraction Preservative: 15 mM sodium azide
Conjugation	PE
Storage	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Gene Name	CD79a molecule
Database Link	Entrez Gene 973 Human UniProt ID P11912
Background	CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
Synonyms	IGA, MB1, B-Cell marker
Reference Data