CD8a (Ly2, T cell Co-receptor, Leu2, T8) (FITC)

Referência C2259-29G-500ug

Tamanho : 500ug

Marca : US Biological

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C2259-29G CD8a (Ly2, T cell Co-receptor, Leu2, T8) (FITC)

Clone Type
Polyclonal
Host
mouse
Source
chicken
Isotype
IgG2b,k
Grade
Purified
Applications
FC IHC IP
Crossreactivity
Ch
Shipping Temp
Blue Ice
Storage Temp
-20°C

In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1,2 Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCR-gamma/delta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-gamma/delta CD8alpha/beta cells in the chicken that is not found in rodents.3||Applications:|• Flow cytometry 4|• Immunohistochemistry (acetone-fixed, frozen tissue sections)|• Purification of CD8+ T cells by positive cell sorting |• Immunoprecipitation 4||Recommended Dilution:|Flow Cytometry: ≤ 1ug/10e6 cells|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Mab|Isotype: IgG2b,k|Clone No: 3-298|Host: mouse|Source: chicken|Concentration: ~0.5mg/ml|Form: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.|Purity: Purified|Specificity: Recognizes the alpha chain of chicken CD8 (Mr 34kD). Species Crossreactivity: Turkey.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
Purity
Purified
Specificity
Recognizes the alpha chain of chicken CD8 (Mr 34kD). Species Crossreactivity: Turkey.
References
1. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133-2138.|2. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249.|3. Tregaskes, C.A., F. Kong, E. Paramithiotis, C.-L. Chen, M.J.H. Ratcliffe, T. F. Davison, and J.R. Young. 1995. J. Immunol. 154:4485-4494.|4. Li, Z., K.E. Nestor, Y.M. Saif, Z. Fan, M. Luhtala, and O. Vainio. 1999. Poult Sci. 78:1526-1531.