CDK5, Active Human Recombinant Protein (p35)

Referência 171848-10ug

Tamanho : 10ug

Marca : US Biological

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171848 CDK5, Active Human Recombinant Protein (p35)

Clone Type
Polyclonal
Swiss Prot
Q00535 (CDK5), Q15078 (p35)
Grade
Purified
Accession #
NM_004935 (CDK5), NM_003885 (p35)
Shipping Temp
Dry Ice
Storage Temp
-70°C

Recombinant full-length human CDK5 (59kD) and human p35 (60kD), both with N-terminal GST tags, were co-expressed in Sf9 cells using a baculovirus expression system. The CDK5 gene accession number is NM_004935 and p35 gene accession number is NM_003885. ||Source:|Full length recombinant protein corresponding to human CDK5 at N-terminal, fused to GST tag expressed in Sf9 cells using a baculovirus expression system.||Molecular Weight: |~59kD, 49kD||Specific Activity: |180pmole/min/ug ||Assay conditions: |Assay was done in Kinase buffer containing 0.2mM DTT using Histone 1 substrate (0.2mg/ml) and 20mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred. ||Application: |Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.||Storage and Stability:|Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Source: Recombinant, Baculovirus|Purity: ~60% |Concentration: As reported |Form: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT, 20% glycerol.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT, 20% glycerol.
Purity
~60%