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CHAPS

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Referência CD0110-1g

Tamanho : 1g

Marca : Bio Basic

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CHAPS
CAS: [75621-03-3]
Grade: Ultra Pure
Storage: 2 to 8C
Product Description: CHAPS

Molecular Formula: C32H58N2O7S
Molecular Weight: 614.9

CHAPS is a standard reagent has been found suile for use as a solubilizing agent in isoelectric focusing applications. It is tested to meet low metal content specifications.

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent for solubilizing membrane proteins and breaking protein-protein interactions. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis, especially for nondenaturing (without urea) IEF.

CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 1-4% (w/v) are typically used in an IEF gel. A commonly used IEF sample solution consists of 8 M urea, 4% CHAPS, 50-100 mM dithiothreitol (DTT), and 40 mM Tris. Furthermore, its small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allow it to be removed from samples by dialysis
Total Product Size:
CD0110 (1g)
CD0110 (5g)
Number of Containers: 1
Refrigeration Requirements: Refrigerator
Shipping Conditions: ICE
UNSPSC Code: 12161902
UNSPSC Category: Detergents/Surfactants
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