Chondroitin Sulfate Proteoglycan, Brain Core Protein (CSPG) (FITC)

The NG2 chondroitin sulfate proteoglycan is a valuable marker for many types of immature progenitor cells including pericytes/smooth muscle cells, chondroblasts and oligodendrocyte progenitors. NG2 is often expressed in melanomas, chondrosarcomas, glioblastomas and myeloid leukemias. Studies indicate that NG2 is involved in modulating cellular response to growth factors 3-5 and cell motility; these are critical functions in proliferation and migration of immature progenitor cells and tumor cells. Oligodendrocyte precursor cells (OPCs) are a population of NG2 expressing cells in the brain that accumulate in response to CNS injuries. OPCs, together with microglia and astrocytes, form the glial scar, which is believed to inhibit or prevent axonal regeneration partly by producing growth inhibiting NG2 at the injury site8. It has been suggested that inhibiting NG2 at sites of spinal cord injury may increase beneficial axonal regeneration. Studies suggest that NG2 stimulates tumor growth and neovascularization in glioblastoma multiforme by inhibiting angiostatin and thus promoting angiogenesis. As NG2 is associated with increased tunmor cell proliferation, it may be a potential therapeutic marker in brain tumors.||Applications:|Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications have not been tested.||Recommended Dilutions:|Immunohistochemistry: 4% paraformaldehyde fixed frozen tissue|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.