K1025-500 | Direct Mouse Genotyping Kit Clinisciences

Product Citation

1. Han Zou, Bradley Poore, et al. "A neurodevelopmental epigenetic programme mediated by SMARCD3–DAB1–Reelin signalling is hijacked to promote medulloblastoma metastasis." Nat Cell Biol. 2023 Mar;25(3):493-507. PMID: 36849558
2. Xiao-Lie He, Li Yang, et al. "Solid lipid nanoparticles loading with curcumin and dexanabinol to treat major depressive disorder." Neural Regen Res. 2021 Mar;16(3):537-542. PMID: 32985484
3. Xiaolie He, Li Yang, et al. "Indispensable CBR1 in Major Depressive Disorder Therapy by Functionalized Solid Lipid Nanoparticles." Preprints. 3 July 2019.

Description

The Direct Mouse Genotyping Kit for genotyping provides convenience in faster preparation and superior PCR amplification. Lysis buffer and balance buffer could digest mice tissues rapidly to release unbroken genomic DNA, and it could be used as PCR template directly without extraction from the mixed solution. By using our kit, you can substantially shorten your time for digestion. Furthermore, the 2X PCR Master Mix (With Dye) will guarantee accurate amplification consequence.

K1025_1

Quality Control

Protocol

Related Biological Data

Related Biological Data

Components and Storage

Complete Kit	200 rxns	500 rxns
Lysis buffer	20 mL	50 mL
Balance buffer	20 mL	50 mL
2X PCR Master Mix (With Dye)	1 mL x 2	1 mL x 5
Proteinase K	200 μL	500 μL
1. Lysis buffer and Balance buffer should be stored at 4°C. 2. 2X PCR Master Mix could be stored at -20°C for 2 years. 3. The Protease K solution should be stored at -20°C and could be stored at 4°C for a short time. Avoid repeated freeze/thaw cycles. Upon first use, it is recommended to aliquot sample into single use portions.

Storage

Lysis buffer and Balance buffer should be stored at 4°C. 2X PCR Master Mix could be stored at -20°C for 2 years. The Protease K solution should be stored at -20°C and could be stored at 4°C for a short time. Avoid repeated freeze/thaw cycles.

Direct Mouse Genotyping Kit

Referência K1025-500

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Marca : APExBIO Technology

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Product Citation

Description

Quality Control

Related Biological Data

Related Biological Data

Components and Storage

Storage

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