Alpha-ketoglutarate dehydrogenase complex is a rate-regulating enzyme in the Krebs Cycle in mitochondria. Dihydrolipoamide succinyltransferase (DLST) is a key subunit in this complex. Mutations in the DLST gene may be responsible for the familial Alzheimer disease. Reduction of DLST increases the generation of reactive oxygen species production suggesting its role in oxidative stress. Deficiency of DLST in mice was suggested to cause increased oxidative stress in mitochondria and is involved in the pathogenesis of Alzheimer disease.||Applications: |Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.||Recommended Dilutions:|Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.|Immunoprecipitation: 1:50|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap