EGFRvIII (Epidermal Growth Factor Receptor, Variant III) (GFR/2600R), Biotin conjugate, 0.1mg/mL

EGFRvIII (Epidermal Growth Factor Receptor, Variant III) (GFR/2600R), Biotin conjugate, 0.1mg/mL

Referência BNCB2600-100

Tamanho : 100uL

Marca : Biotium

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Antibody number

#2600

Antibody reactivity (target)

EGFRvIII

Antibody type

Primary

Host species

Rabbit

Clonality

Recombinant Monoclonal

Clone

GFR/2600R

Isotype

IgG

Molecular weight

~145 kDa (vIII variant)

Synonyms

Erbb1 variant III; ERBB1 variant III; HER1 variant III; Proto-oncogene c-ErbB-1 variant III; Receptor Tyrosine Protein Kinase variant III; ErbB1 variant III

Human gene symbol

EGFR

Entrez gene ID

1956

SwissProt

P00533

Unigene

488293

Immunogen

KLH conjugate of EGFRvIII-specific, 14-amino acid peptide from the predicted amino acid sequence at the fusion junction (LEEKKGNYVVTDHC)

Antibody target cellular localization

Plasma membrane

Verified antibody applications

IHC (FFPE) (verified)

Species reactivity

Human

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user

Positive control

A431 cells. Placenta, Breast, Colon or Bladder Carcinoma

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Cancer, Signal transduction

Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Tumor expression

Squamous cell carcinoma

Protocols
SDS

Download Datasheet

Product Description

EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the

CF® Dye Brochure

for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email

info@clinisciences.com

to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 2600, Anti-EGFRvIII (GFR/2600R) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405

CF®405S Features

BNC88 CF®488A 490/515 488 GFP, FITC

CF®488A Features

BNC68 CF®568 562/583 532, 561 RFP, TRITC

CF®568 Features

BNC94 CF®594 593/614 561 Texas Red®

CF®594 Features

BNC40 CF®640R 642/662 633-640 Cy®5

CF®640R Features

BNC47 CF®647 650/665 633-640 Cy®5

CF®647 Features

BNC74 CF®740 742/767 633-685 775/50

CF®740 Features

BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free
N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.