Erythrocytes Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, R, WB
Recommended Dilution	ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, Guinea Pig complement (at a 1/60 dilution) and 2% RBC, not less than 1/500 when tested with Bovine Erythrocytes.
Reactivities	Bovine
Host	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	Washed whole Erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Bovine Erythrocytes. To sensitize cells in Haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes. Homologous proteins of different species frequently share antigenic determinants. Cross-reactivity has not been tested.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized purified Hyperimmune IgG fraction
Reconstitution Method	Restore by adding 1.0 ml of sterile distilled water
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Unconjugated
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data