ExoTrap Exosome Isolation Spin Column Kit for protein research

Exosome are cell-derived vesicles formed by a lipid bilayer membrane, and its diameter is 40-100 nm. Exosomes are observed in body fluid, such as saliva, blood, urine, amniotic fluid, malignant ascites, and are secreted from cultured cells. Recently, it has been shown that exosomes include various proteins and RNAs, which have a possibility to function in intercellular signal transduction. These products are the antibodies which can specifically detect CD9, CD63 and CD81 known as exosome markers. These antibodies are suitable for isolation of exosome by immunoprecipitation.

-High specificity for exosome membrane proteins CD9, CD63, CD81

-100% of exosomes in a 150 ul sample can be isolated by using only 1 ug of antibody
Supported samples:
CD9…serum
CD63…serum, culture supernatant
CD81…serum, culture supernatant

-Useful for analyses of exosome surface antigen proteins, endogenous RNA (miRNA) and proteins

1) IP-WB of exosome in serum sample using Anti-CD9 antibody (12A12)

2) IP-WB of exosome in serum sample using Anti-CD63 antibody (8A12)

3) IP-WB of exosome in serum sample using Anti-CD81 antibody (12C4)