F13B, NT (F13B, Coagulation factor XIII B chain, Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain) (Azide free) (HRP)

Referência 035305-HRP-200ul

Tamanho : 200ul

Marca : US Biological

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035305-HRP F13B, NT (F13B, Coagulation factor XIII B chain, Fibrin-stabilizing factor B subunit, Protein-glutamine gamma-glutamyltransferase B chain, Transglutaminase B chain) (Azide free) (HRP) discontinued

Clone Type
Polyclonal
Host
rabbit
Source
mouse
Swiss Prot
P05160
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Mo
Accession #
NP_001985.2
Gene #
F13B
Shipping Temp
Blue Ice
Storage Temp
-20°C

This gene encodes coagulation factor XIII B subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.||Applications:|Suitable for use in Western Blot, ELISA||Recommended Dilution:|ELISA: 1:1,000|Western Blot: 1:100-500||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography.|Immunogen: F13B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the N-terminal region of human F13B.|Specificity: Mouse||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
F13B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the N-terminal region of human F13B.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Mouse
References
Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010)|Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :|Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)|Romero, R., et al. Am. J. Obstet. Gynecol. 202 (5), 431 (2010) :|Davila, S., et al. Genes Immun. 11(3):232-238(2010)