ASM10531-A633-100 | FIP200 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FIP200 Polyclonal Antibody (ASM10531). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FIP200 Polyclonal Antibody (ASM10531) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus, Cytoplasm, Cytoplasm, Cytosol, Preautophagosomal Structure. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FIP200 Antibody (D) Composite.
Western blot analysis of Rat Brain cell lysates showing detection of 183 kDa FIP200 protein using Rabbit Anti-FIP200 Polyclonal Antibody (ASM10531). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FIP200 Polyclonal Antibody (ASM10531) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 183 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	Q8TDY2
Other Accession	NP_001077086.1
Host	Rabbit
Reactivity	Human, Rat
Clonality	Polyclonal
Description	Rabbit Anti-Human FIP200 Polyclonal
Target/Specificity	Detects ~150 kDa.
Other Names	RB1CC1 Antibody, FAK family kinase-interacting protein of 200 kDa Antibody, FIP200 Antibody, KIAA0203 Antibody, RBICC Antibody, RB1-inducible coiled-coil protein 1 Antibody
Immunogen	Synthetic peptide from the C-terminal of Human FIP200 (aa. 1355-1366)
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-658 was sufficient for detection of FIP200 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Nucleus \| Cytoplasm \| Cytoplasm \| Cytosol \| Preautophagosomal Structure

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