K2174-100 assays | Glutathione Peroxidase Activity Colorimetric Assay

Background

Glutathione Peroxidase (GPx) family of enzyme protects cell from oxidative stress. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Low levels of GPx have been linked to free radical related disease. In the Glutathione Peroxidase Activity Colorimetric Assay, GPx reduces Cumene Hydroperoxide as well as oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease NADPH can be measured at 340 nm and is proportional to GPx activity. The assay can be used to detected glutathione dependent peroxidaes in tissue homogenates, plasma and cell lysates. The detection limit is approx. 0.5 mU/mL of GPx in samples.

Features & Properties

Features	Simple procedure; Takes approx. 30-40 minutes; Fast and convenient.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

GPx Assay Buffer, NADPH (lyophilized), Glutathione Reductase, Glutathione (GSH, lyophilized), Cumene Hydroperoxide, GPx Positive Control (lyophilized)

Glutathione Peroxidase Activity Colorimetric Assay Kit

Referência K2174-100 assays

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Marca : APExBIO Technology