Goat anti-ERV3
Referência EB10499
Tamanho : 100ug
Marca : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB10499 | Goat Anti-ERV3 Antibody | Pep-ELISA, WB, IHC | 100µg specific antibody in 200µl | Human |
Target Protein
Principal Names: endogenous retroviral sequence 3, endogenous retroviral sequence 3 (includes zinc finger protein H-plk/HPF9), envelope polyprotein, ERV-3 envelope protein, ERV-R, HERV-R, H-PLK, ERV3Official Symbol: ERV3-1
Accession Number(s): NP_001007254.2
Human GeneID(s): 2086
Immunogen
Peptide with sequence C-PQDNFTLTASS, from the internal region of the protein sequence according to NP_001007254.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:4000.Western blot: This product has been successfully used in WB on Human: Nahas MR et al, Br J Haematol. 2019 May;185(4):679-690. PMID: 30828801.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 3.75µg/ml. This product has been successfully used in IHC on Human: Frese S et al, PLoS One. 2015 Jul 8;10(7). PMID: 26154387.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human
Specific References
This antibody has been successfully used in Western blot on Human:
Nahas MR, Stroopinsky D, Rosenblatt J, Cole L, Pyzer AR, Anastasiadou E, Sergeeva A, Ephraim A, Washington A, Orr S, McMasters M, Weinstock M, Jain S, Leaf RK, Ghiasuddin H, Rahimian M, Liegel J, Molldrem JJ, Slack F, Kufe D, Avigan D
Hypomethylating agent alters the immune microenvironment in acute myeloid leukaemia (AML) and enhances the immunogenicity of a dendritic cell/AML vaccine
Br J Haematol. 2019 May;185(4):679-690.
PMID: 30828801
This antibody has been successfully used in IHC on Human:
Frese S, Ruebner M, Suhr F, Konou TM, Tappe KA, Toigo M, Jung HH, Henke C, Steigleder R, Strissel PL, Huebner H, Beckmann MW, van der Keylen P, Schoser B, Schiffer T, Frese L, Bloch W, Strick R.
Long-Term Endurance Exercise in Humans Stimualtes Cell Fusion of Myoblasts along with Fusogenic Endogenous Retroviral Genes In Vivo.
PLoS One. 2015 Jul 8;10(7).
PMID: 26154387
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol