Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) - AF488

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Referência CSA3208-1ml

Tamanho : 1ml

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Telefone : +1 850 650 7790

Marca : Cohesion Biosciences

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

Line colors represent the approximate visible colors of the wavelength maxima.
Immunofluorescent analysis of beta Tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST. The secondary antibody (green) was Goat Anti-Mouse IgG (H&L) - AcalephFluor488 in PBS at room temperature in the dark.

Product Name:	Goat Anti-Mouse IgG (H&L) - AcalephFluor488
Cat No:	CSA3208
Source:	Goat
Reactivity:	M
Applications:	E, IH, IF, FC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) - AcalephFluor488

Immunogen：Mouse IgG

Purification：The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Clonality：Polyclonal

Conjugation：AcalephFluor488

Form：1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide

Dilution：IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Platform：Ex/Em = 490/515 nm

Human Astrocyte Spheroids as Suitable In Vitro Screening Model to Evaluate Synthetic Cannabinoid MAM2201-Induced Effects on CNS

Journal Int. J. Mol. Sci

IF 6.208

Application IF

Reactivity

PMID 36674936

Recombinant growth arrest-specific protein 6 protects the blood–brain barrier by regulating microglia polarization via the GAS6/Axl/SOCS pathway in post-stroke hemorrhagic transformation model

Journal Research

IF 11

Application

Reactivity

PMID

MAM-2201, One of the Most Potent-Naphthoyl Indole Derivative-Synthetic Cannabinoids, Exerts Toxic Effects on Human Cell-Based Models of Neurons and Astrocytes

Journal Neurotoxicity Research

IF 3.911

Application IF

Reactivity

PMID 33945101

Intelligent Supramolecular Modification for Implants: Endogenous Regulation of Bone Defect Repair in Osteoporosis

Journal Advanced Materials

IF 27.4

Application IF

Reactivity

PMID 39166701

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