Green Gel Loading Buffer with DNA Stain

Gel Loading Buffer with DNA Stain (l.) vs.
gel stained with Ethidium Bromide (r.)

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
store dark

Shelf Life: 12 months

Form: Liquid

Concentration: 6x conc.

Applications:
Green Gel Loading buffer with DNA Stain is recommended for analyzing DNA fragments 100 - 2000 bp.

Add one part of Gel Loading Buffer to 5 parts of the DNA sample as shown in the table below. Spin down the tubes and vortex gently to achieve homogeneity.

Description:
Jena Bioscience Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffer contains a green-fluorescent DNA stain specially developed for DNA analysis applications. The high quantum yield and excellent stability make the dye the ideal fluorophore for DNA staining applications and an excellent replacement for the widely used dye ethidium bromide.
The buffers contain tracking dyes as indicator for DNA fragment migration. In addition, they contain glycerol to add density and EDTA to inhibit nuclease activities.
The buffers are optimized for loading of DNA fragments in a size range of:

6x Gel Loading Buffer:
60 mM Tris-HCl (pH 7.5), 60 mM EDTA, 50 % (w/v) Glycerol, xylene cyanol FF, Tartrazine, green-fluorescent DNA stain

Performance:
The Gel Loading Buffer provides highest convenience during routine handling and avoids commonly used gel staining procedures with Ethidium Bromide.

Fragment Separation on Agarose Gels:

BIOZ Product Citations:

Powered by Bioz See more details on Bioz