Green Gel Loading Buffer with DNA Stain
Referência PCR-275
Tamanho : 5x1,8ml
Marca : Jena Bioscience
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Loading buffer for agarose or polyacrylamide gels with SYBR® Green fluorescent DNA stain
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
store dark
Shelf Life: 12 months
Form: Liquid
Concentration: 6x conc.
Applications:
Green Gel Loading buffer with DNA Stain is recommended for analyzing DNA fragments 100 - 2000 bp.
Add one part of Gel Loading Buffer to 5 parts of the DNA sample as shown in the table below. Spin down the tubes and vortex gently to achieve homogeneity.
Description:
Jena Bioscience Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain SYBR® Green DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes SYBR® Green the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide.
The buffers contain tracking dyes as indicator for DNA fragment migration. In addition, they contain glycerol to add density and EDTA to inhibit nuclease activities.
The buffers are optimized for loading of DNA fragments in a size range of:
6x Gel Loading Buffer:
60 mM Tris-HCl (pH 7.5), 60 mM EDTA, 50 % (w/v) Glycerol, xylene cyanol FF, Tartrazine, SYBR® Green
Performance:
The Gel Loading Buffer provides highest convenience during routine handling and avoids commonly used gel staining procedures with Ethidium Bromide.
Fragment Separation on Agarose Gels:
BIOZ Product Citations: