H-Glu(EDANS)-Pro-Leu-Phe-Ala-Glu-Arg-Lys(DABCYL)-OH [1914987-47-5]

Internally quenched substrate for calpain-1 (µ-calpain) with optimal cleavage motifs flanking the scissile bond. The enzyme showed a more than 18-fold higher turnover rate for the hydrolysis of this FRET substrate based on the amino acid sequence PLFAER than for EVYGMM a sequence derived from the cleavage site of the natural substrate α-spectrin.