Reagentes e instrumentos para imunologia, biologia celular e biologia molecular.

HCG-alpha(HCGa/53), 0.2mg/mL

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Referência BNUB0053-100

Tamanho : 100uL

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Telefone : +1 850 650 7790

Marca : Biotium

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

HCG Alpha Monoclonal Mouse Antibody (HCGa/53)

Product Attributes

Antibody number	#0053
Antibody reactivity (target)	HCG Alpha
Antibody type	Primary
Host species	Mouse
Clonality	Monoclonal
Clone	HCGa/53
Isotype	IgG1, kappa
Molecular weight	~13 kDa
Synonyms	CG-alpha; CGA; Chorionic Gonadotrophin Alpha; Follicle Stimulating Hormone Alpha; Follitropin Alpha; FSH-alpha; FSHA; GPH Alpha; GPHA1; LHA; LH-alpha; Luteinizing Hormone Alpha; Lutropin Alpha; Thyroid Stimulating Hormone Alpha; Thyrotropin Alpha; TSHA
Human gene symbol	CGA
Entrez gene ID	1081
SwissProt	P01215
Unigene	119689
Immunogen	Recombinant hCG alpha protein
Verified antibody applications	IHC (FFPE) (verified)
Antibody target cellular localization	Secreted (extracellular), Vesicular
Species reactivity	Human
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology formalin-fixed 1-2 ug/mL, No special pre-treatment is required for staining of formalin-fixed tissues. Note that HIER is NOT recommended for this antibody, Optimal dilution for a specific application should be determined by user
Positive control	JAR or TT Cells. Placenta
Shipping condition	Room temperature
Storage Conditions	Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life	Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status	For research use only (RUO)
Antibody/conjugate formulation	Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas	Endocrinology
Product origin	Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression	Placenta

Technology

CF® Dyes

Considerations for Immunofluorescence Staining

Primary & Secondary Antibody Conjugates

Product Description

This MAb reacts with a protein of ~13 kDa, identified as α sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@clinisciences.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 0053, Anti-HCG Alpha (HCGa/53)

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNC74	CF®740	742/767	633-685	775/50	CF®740 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.